【摘要】 以种鸡蛋为实验材料 ,分别在孵化 0、3和 6d后 ,抽取 1mL蛋清 ,用石蜡或自配封口胶封闭针孔 ,以探索抽蛋清和不同封口方法对不同日龄鸡胚发育的影响。实验结果表明 ,抽取蛋清对 3日龄鸡胚的发育和孵出的影响较小 (P <0 .0 1) ,孵化率达 76 .3% ,而对 0日龄、6日龄鸡胚发育和孵出的影响较大 ,孵化率只有 30 .6 %和 4 1.2 %。与石蜡封口相比 ,自配封口胶封口组的鸡胚孵化率明显提高 (P <0 .0 1) ,自配封口胶封口组的鸡胚孵化率为 76 .3% ,石蜡封口组的鸡胚孵化率只有 2 1.4 %。更多还原

【Abstract】 The factors influencing the in vitro culture of chicken embryos was made by taking 1 mL egg white with a syringe and sealing the pinprick with paraffin wax and our sealing gum. The results indicate that the hatching rate in 3 days chicken embryos by taking 1 mL egg white is 76.3% (P<0.01), while that in 0 day and 6 days chicken embryos is 30.6% and 41.2% respectively. And the hatching rate in 3 days chicken embryos sealed with our sealing gum is 76.3% (P<0.01), while that in 3 days chicken embryos sealed with paraffin wax is 21.4%.更多还原