【摘要】 目的探讨应用显微外科技术建立大鼠胰十二指肠移植模型的手术技巧。方法以雄性SD大鼠为供受体。对供体于进腹后采用连续滴注法经腹主动脉灌注4℃复方乳酸林格液7～8ml,同时整块切取带全胃、部分结肠在内的供体后再修剪。对供体门静脉与受体左肾静脉行袖套吻合,对供体腹主动脉与受体腹主动脉行端侧吻合,对供体十二指肠与受体近端空肠行侧侧吻合。结果62只经药物诱导的糖尿病大鼠移植术后存活超过24h者56只,其中54只血糖降至正常水平。结论熟练、扎实的显微外科技术是建立大鼠十二指肠移植模型的基础;该模型稳定性强,可重复性好,是临床、基础研究的理想模型。更多还原

【Abstract】 Objective To investigate microsurgical techniques in establishing rats pancreasticodudenal transplantation.Methods Male SD rats were served as donors and recipients.The donor pancreaticodudodenal was excised with en bloc technique while a fine needle with infusion was introduced into the aorta distal and perfusion was conducted continiously until bloodless with 7～8 ml of cold (4℃)saline,then the graft including stomach and part colon was clipped.The portal venous of the graft was anastomosed with the recipent’s left renal vein by a cuff technique.End to side anastomosis was performed for artery employing 8-0 nylon sutures,a side to side anastonosis was made between the graft duodenum and the host jejunum.Results Of 62 transplanted rats with induced diabetes,the survival time of 56 rats was over 7 days,moreover,the blood sugar level of 54 rats descended to normal.Conclusions It is essential with well tried microsurgical techniques in establishing a model of rats pancreaticoduodenal transplantation.This model can be performed repeatedly,and it is a tested model for clinical or basic studay.更多还原