【摘要】 目的评价免疫印迹法(IB)检测可提取核抗原(ENA)多肽抗体的性能。方法以检测抗ENA抗体中的抗U1RNP抗体为研究对象,用免疫印迹法(IB)和免疫双扩散法(ID)分别进行检测,以高特异的ID法检测结果为对照,统计IB法的符合率。结果用IB法检测抗U1RNP抗体有7种不同的蛋白质排列组合,其中以73,32,17.5kDa三条蛋白带同时出现与ID法的符合率最高(84.1%),而32kDa的符合率最低(30.7%),这两组的差异最显著(P<0.01)。结论单独采用IB法检测抗ENA抗体尚欠可靠,须结合其它方法,取长补短,提高ENA抗体检测的敏感性、特异性。更多还原

【Abstract】 Objective To estimate the capability of immuno bl otting(IB) detection of extractable nuclear antigen (ENA) polypeptide autoantibo dy.Methods Detection of anti-U1RNP antibodies in ENA aut oantibody as study object,detection was carried out both in immunoblotting techn ique (IB) and immunodiffusion (ID).The very specific ID detection result was com pared.The coincidence rate of IB was counted.Results Ther e were seven different protein combinations with immunoblotting detection U1RNP antibodies.Protein zones of 73 kDa,32 kDa and 17.5 kDa appeared simultaneity,and the coincidence rate (84.1%) with ID was the maximun.While the 32 kDa one with ID was the minimun.The defference between these groups was the most obvious (P <0.01).Conclusion It is lack of trustiness to detect E NA autoantibody by using IB singly.To enhance the indexes of sensitivity and spe cific,it is essential be combined with other methods.更多还原