【摘要】 利用60Co-γ射线(辐照剂量为300Gy,剂量率为4.6875Gy/min)辐照豆科决明属5个牧草品种的种子,通过盆栽收集各个品种的M1代种子,然后进行M2代试验与观察。结果表明,辐照后5个决明M2代的种子出苗率、植株的茎长、叶宽均无显著差异;34721号决明植株冠高有显著增长、2219号决明茎径有显著增长、86134号决明分枝数有极显著增长,92985号决明分枝数则有显著降低、86134号决明叶数和地上部植株干重有显著增长、2219号决明叶长增长显著,其余参试品种则无显著差异。植株的营养成分测定表明:仅2219号决明K含量呈显著下降、其他品种的N,P,K含量则无显著差异;2219不仅氨基酸总量呈显著下降、而且17种氨基酸都表现为不同程度的下降,86134氨基酸总量有显著增长、同时17种氨基酸都表现为不同程度的增长,而其余3个参试品种都无显著差异。经60Co-γ射线辐照处理后的34721,86134,92985的酯酶同工酶谱与相应的对照有明显差别。生长观察结果表明,参试的5个决明品种M2代植株生长的现蕾期、初花期、盛花期、初荚期、盛荚期、成熟期表现为双向性,既有提早,也有推迟。更多还原

【Abstract】 The seeds of 5 accessions of Chamaecrista were irradiated by 60Coγ-ray (radiation dosage: 300 Gy, radiation rate: 4.6875 Gy/min). The M1 seeds were collected from the potted plants, and then the M2 were tested and observed. The results showed that the differences of the germination rate, stem length, leaf width of the 5 varieties of Chamaecrista were not significant. However, the height of Chamaecrista No. 34721 and the stem diameter of No. 2219 increased significantly. The branch number of Chamaecrista No. 86134 increased significantly (P<0.01) while that of No. 92985 decreased significantly (P<0.01). The leaf number and the dry weight of the plants above the soil of No. 86134 and the leaf-length of No. 2219 increased significantly. There were no significant differences in other varieties. The nutrition determination results showed that K content of No. 2219 decreased significantly, and that there were no significant differences in N, P and K contents in the other 4 varieties. Not only the total amino acid content of No. 2219 decreased significantly, but also the 17 amino acids decreased in various degrees. Not only the total amino acid content of No. 86134 increased significantly, but also the 17 amino acids increased to various extents. There were no significant differences in the other 3 varieties. The zymograms of esterase isozyme of the irradiated accessions No. 34721, 86134 and 92985 were significantly different from those of the CKs. Growth observations showed that the M2 plants of all the varieties under observation changed the stages of floral bud shooting, early flower, full flower, initial pod, full pod and mature pod, and turned to be early or late varieties.更多还原