【摘要】 目的探讨细胞间粘附分子(ICAM-1)表达与中性粒细胞(PMNs)浸润、缺血再灌注损伤的关系,并观察水飞蓟宾-卵磷脂复合物(silybin-lecithin compound,SLC)对缺血再灌注损伤的保护作用机理。方法蒙古沙土鼠155只。分设:1)缺血组;2)缺血再灌注组;3)SLC100mg/kg组;4)SLC200mg/kg组;5)SLC400mg/kg组;6)假手术对照组;7)溶剂组。除假手术对照组外,每组分设1、3、6、12、24h时相点。取鼠脑组织块用原位杂交和免疫组化检测方法测定ICAM-1mRNA及其蛋白表达水平,用酶法测定PMNs浸润数。结果脑缺血再灌注时,ICAM-1表达明显增高,其中ICAM-1mRNA表达至6h达高峰,而ICAM-1蛋白质表达水平、PMNs浸润数改变于12~24h达最高峰,二者之间呈显著正相关,但ICAM-1mRNA与后二者间无明显相关性。SLC组上述指标于再灌注时显著下降,其下降程度与时相和浓度呈明显的负相关。均P<0.01或0.05。结论脑缺血再灌注时,ICAM-1参与介导了PMNs对组织细胞的粘附、浸润和再灌注损伤的发生发展,SLC可通过抑制ICAM-1的表达而产生神经细胞的保护作用。更多还原

【Abstract】 Objective To investigate the influence of expression of intercellular adhesion molecule-1(ICAM-1) on cerebral ischemic reperfusion injury and cerebral infiltration of polymorphonuclear neutrophils (PMNs).Methods using an established common carotid artery occlusion model,we observed the expression of ICAM-1 by in setup hybridization and the protein by immunocytochemistry.and the numbers of infiltrated PMNs were measured with MPO enzyme marker method.Results After cerebral ischemic reperfusion,cerebral levels of expression of ICAM-1 were increased significantly and reached a peak at 6h;but the ICAM-1 protein peaked during 12-24h.Number of PMNs also increased and peaked after reperfusion 12-24h.Close positive correlation (P<0.05) was found between the levels of expression of ICAM-1 and PMNs.In SLC group,all of the indices were increased after ischemic reperfusion,but the levels of increase were significantly lower(P<0.05 or P<0.01) as compared with PVP group.Conclusions The findings indicate that ICAM-1 and PMNs is implicated in the development of cerebral ischemic reperfusion injuries.SLC could protect the cerebral tissue from cerebral ischemic reperfusion by partially blocking the expression of ICAM-1.更多还原