【摘要】 目的探讨脑缺血再灌注损伤不同缺血区域的炎症反应。方法将雄性Wistar大鼠85只,随机分为脑缺血再灌注组(70只)和假手术组(15只)。前者用线栓法建立局灶性脑缺血再灌注模型,脑缺血90min再灌注,将大鼠随机分为7个时间点,分别是再灌注后3、6、12、24、48、72和96h,每个时间点10只大鼠。采用免疫组化法和生化法,观察额顶部皮质和基底核区P选择素表达、髓过氧化物酶(MPO)活性变化。结果①再灌注后缺血侧微血管内皮细胞表面出现P选择素表达,主要分布于额顶部皮质和基底核区:3h额顶部皮质为(13±4)支,基底核区为(11±3)支;12h额顶部皮质为(60±6)支,基底核区为(57±5)支;72h额顶部皮质为(19±4)支,基底核区为(16±4)支。②再灌注后12、24、48、72和96h各时间点MPO活性:额顶部皮质分别是(0.206±0.029)U/g、(0.421±0.030)U/g、(0.369±0.022)U/g、(0.341±0.023)U/g和(0.328±0.028)U/g;基底核区分别是(0.183±0.013)U/g(0.292±0.017)U/g(0.340±0.012)U/g(0.226±0.018)U/g、(0.158±0.022)U/g。假手术组MPO活性:额顶部皮质为(0.033±0.005)U/g、基底核区为(0.031±0.004)U/g,比再灌注组低(P<0.05)。基底核区的MPO活性在再灌注48h达到高峰后下降。额顶部皮质MPO活性在再灌注24h达到高峰后至96h仍维持较高水平。结论脑缺血再灌注损伤时,存在主动炎症反应:出现P选择素表达和中性粒细胞浸润,额顶部皮质比基底核区的炎症反应更强烈和持久。更多还原

【Abstract】 Objective To study the inflammatory reaction in different ischemic area after focal cerebral ischemia-reperfusion injury in rats. Methods 85 male Wistar rats were randomly assigned into ischemia-reperfusion group (70) and sham operation group (15).The focal middle cerebral artery occlusion (MCAO) model was made by inserting a suture.Reperfusion was made 90 minutes after MCAO.The expression of P-selectin and the Myeloperoxidase(MPO) activity were investigated in the frontal and parietal cortex and basal ganglia with immunohistochemistry at different time points 3,6,12,24,48,72,96 h after reperfusion.HE and TTC staining was also made. Results ①Expression of P-selectin was showed on ischemic endothelium,mainly in the ischemic frontal and parietal cortex and basal ganglia.At 3 h,13 ± 4 vessels with P-selectin expression were found in the frontal and parietal cortex,11 ± 3 in basal ganglia;there were 60 ± 6 and 57 ± 5 in these two areas at 12 h respectively.And 19 ± 4,16 ± 4 at 72h.② MPO activity was showed(0.206 ± 0.029)U/g,(0.421 ± 0.030) U/g,(0.369 ± 0.022)U/g,(0.341 ± 0.023)U/g and(0.328 ± 0.028)U/g at 12,24, 48,72 and 96 h respectively in the frontal and parietal cortex,while(0.183 ± 0.013)U/g,(0.292 ± 0.017) U/g,(0.340 ± 0.012)U/g,(0.226 ± 0.018)U/g and(0.158 ± 0.022)U/g in basal ganglia respectively in ischemia-reperfusion group.These were higher than control group (P < 0.05).In ischemic basal ganglia,MPO activity peaked at 48h,gradually descended afterwards.In the frontal and parietal cortex,MPO activity peaked at 24h,and remained elevated until 96h. Conclusion There is inflammatory reaction after cerebral ischemia-reperfusion injury.P-selectin is expressed,and neutrophils infiltration.The inflammation is more persistent and intensive in ischemic frontal and parietal cortex than the basal ganglia.更多还原