【摘要】 采用非均相反应制备NaCS(硫酸纤维素钠),考察了反应液中正丙醇-硫酸的最佳比例为25∶35,NaCS和PDMDAAC的最适滴制浓度分别为4%和3%;利用NaCS-PDMDAAC微胶囊对假单胞菌TS-1138进行固定化并进行连续培养,结果表明,菌体能在胶囊内很好的生长和繁殖,连续培养132h后菌浓可达到4.5×1011个/mL胶囊,是在相同条件下游离培养的6倍;固定化菌体以ATC为底物生成L-半胱氨酸的催化活性是游离培养菌体的2.5倍,并且固定化菌体的重复使用能力明显优于游离菌体.重新考察固定化菌体的最适催化反应时间为3h,在一定程度上弥补了微胶囊传质性相对较差的不足.更多还原

【Abstract】 Based on the heterogenetic reaction, a process of preparation of NaCS(Sodium Cellulose Sulfate) was proposed. The optimum proportion of optal-sulfuric acid which was studied in all the effects on the synthesis of NaCS was 25∶35. The suitable concentrations of NaCS and PDMDAAC in the course of dropping were 4% and 3% respectively. Pseudomonas sp. TS-1138 was immobilized in the NaCS-PDMDAAC microcapsule and cultured continuously. The experiment showed that the cells could grow well. In 136 hours, the density of cells could be 4.5×10~11个/mL capsule which was 6 folds than that in free cell culture at the same condition, and the enzyme activity from ATC to L-cysteine was 2.5 folds. The microencapsulated cells is clearly superior to the free cells when they were applied repeatedly. The optimum catalytic reaction time by the microencapsulated cells is 3 hours, which partly make up the shortcoming of mass transfer by microcapsule.更多还原