【摘要】 建立了用Source 15Q强阴离子交换柱分离纯化寡聚核苷酸的方法.在pH 9.0,以20 mmol/L Tris-HCl缓冲液+2.0 mol/L NaCl为流动相,线性浓度梯度洗脱,紫外检测波长为260 nm,流速为1 mL/min的分离条件下,能很好地分离18-78 mer的合成寡聚脱氧核苷酸和PCR的单双链产物,探讨了流速和盐浓度对分离的影响,并测得24mer OligoDNA的回收率为90%左右.该方法简便、快捷、分辨率高,可用于寡聚核苷酸、反义核酸药物的分离、纯化、鉴定和制备.更多还原

【Abstract】 The method or separation ot oligodeoxynucleotide by high pertormance anion exchange chro-matography with Source 15Q column was established. The oligodeoxynucleotide ranging from 19 mers to 78 mers can be successfully separated μL by linear gradient elution of 2.0 mol/L sodium chloride in 20 mmol/L Tris-HCl buffer(pH 9.0), UV detected 260 nm, and the flow rate of 1 mL/min. At the same time, the recovery rate of 24 mers oligoDNA about 90% was determined. The influences of pH value, salt concentration and flow fate on separation were discussed, too. This method can also be applied to separate, purify, evaluate and prepare characteristic antisense nucleotide because of its simplicity, fastness, accuracy and high discrim-inability.更多还原