【摘要】 目的:探讨家兔主要淋巴器官肥大细胞(MC)的分布特征与组织化学特点。方法:将家兔脾脏及圆小囊用Carnoy液和 (或)4％中性甲醛溶液(NBF)固定,进行常规甲苯胺蓝染色或长时间甲苯胺蓝染色(LTB),光镜观察。结果:家兔的圆小囊MC 主要分布于黏膜上皮或黏膜下层结缔组织中,脾脏MC则主要分布于红髓淋巴细胞间,两者非胸腺依赖区的淋巴滤泡中均无 MC,Carnoy液固定的家兔组织染色效果更佳,采用LTB染色可不同程度的增加MC的着染性。结论:MC都具有胸腺依赖性或 T细胞依赖性,淋巴器官MC与T淋巴细胞位置关系密切。不同固定液和不同时间染色方法所获得的MC数量不同。更多还原

【Abstract】 Objective: To investigate the distribution and histochemical characteristics of mast cells in major lymphatic organs of rabbits. Methods: Rabbits’ spleen and sacculus rotundus tissues were fixed with Carnoy’s fluid or neutral buffered formalin (NBF) stained by routine toluidine blue or long time toluidine blue (LTB) and then observed under microscopy. Results: Mast cells of sacculus rotundus were mainly distributed in mucosa epithelium or submucosa connective tissues, and those of spleen were located in lymphocytes of red pulp. No mast cell was found in the lymph follicle of thymus independent region in sacculus rotundus or spleen. Staining result of MCs fixed with Carnoy’s fluid was excellent, and staining was enhanced by LTB staining. Conclusion: Mast cells of sacculus rotundus and spleen are thymus dependent or T cell dependent, and have close ties with the location of T lymphocytes. The amount of mast cells is various with different fixtations and staining times.更多还原