【摘要】 目的探讨检测大肠癌患者外周血微转移的免疫磁珠技术的方法及临床意义。方法采集大肠癌患者外周抗凝血5ml,加入6%HES200/0.5,4℃下静置60min,取上清,加入抗CEA单克隆抗体,在4℃下作用90min,然后室温下与免疫磁珠作用60min,在磁性细胞分离器中静置30min,镜下观察玫瑰花环形成情况,HE染色,进行免疫磁珠技术敏感性测定。结果5ml外周血中的肿瘤细胞可被检测出来,大肠癌患者的阳性检出率为42.5%。结论免疫磁珠技术是一种有效检测肿瘤细胞的方法,可以从大肠癌患者外周血中分离出肿瘤细胞,监测微转移的存在,有助于临床对大肠癌的诊断及治疗。更多还原

【Abstract】 Objective To study the method of immunomagnetic beads(IMB) technique for micrometastasis in the peripheral blood of colorectal carcinoma and its clinical significance.Methods The colorectal carcinoma cells in blood samples,from 40 patients,were separated by using 6% HES 200/0.5 to deposit the red blood cells.The mixture including tumor cells were incubated with anti CEA Mab,90 minutes and then reacted with IMB for 60 minutes.The cells were collected in the magnetic cell segregator,observed through microscope and HE dyed,then to evaluate the sensitivity of the technique.Results The malignant cells could be detected in the peripheral blood.Colorectal carcinoma cells were found in 17 of 40 cases,and the detectable rate was 42.5 %.Conclusion This method can be used to detect tumor cells from peripheral blood of patients with colorectal carcinoma and monitor micrometastasis,therapeutic protocol and prediction of prognosis can be made based on it.更多还原