【摘要】 目的探讨甘利欣对酒精性肝病的治疗作用。方法Wistar鼠随机分为正常对照组、模型组、未治组和治疗组,56°白酒灌胃制造酒精性肝病模型,治疗组腹腔注射甘利欣,未治组腹腔注射生理盐水做对照;腹主动脉采血检测肝功、ELISA法检测肿瘤坏死因子(TNFα)水平,取肝脏组织做病理组织学检查。结果正常对照组肝脏病理均为正常,模型组酒精性肝病按病理分级,4级13例,3级2例,2级1例;未治组(4级15例,3级3例,2级2例)与模型组无明显差异,P>0.05;治疗组正常3例,1级10例,2级5例,与未治组比较P<0.05;治疗组丙氨酸氨基转移酶(ALT)(36.3U/L±11.5U/L)、天门冬氨酸氨基转移酶(AST)(46.7U/L±18.2U/L)、γ谷胺酰转肽酶(γGT)(55.3U/L±12.3U/L)、TNFα(1.6ng/ml±0.52ng/ml)明显低于模型组和未治组(ALT198.1U/L±66.3U/L,189.0U/L±69.7U/L,AST253.0U/L±62.1U/L,268.0U/L±60.5U/L,γGT156.6U/L±38.7U/L,149.8U/L±38.1U/L,TNFα2.8ng/ml±0.65ng/ml,2.7ng/ml±0.60ng/ml),P<0.05。结论甘利欣通过降低血清TNFα水平,改善肝组织治疗酒精性肝病。更多还原

【Abstract】 Objective To investigate the effect of the treatment of alcoholic liver disease with alprostadil.Methods Wistar rats were divided randomly into normal control,model,untreated and treated groups,56° spirit was used to make alcoholic liver disease models,treated group was injected with alprostadil intraperitoneally;while normal and model groups with saline for control,extracting blood from abdominal aorta for biochemical examination and detecting the level of TNF α with ELISA,sampling liver tissue for pathological examination.Results Liver pathology showed that all parameters in the normal control group were normal,model group:13 cases with class 4,2 cases with class 3,1 case with class 2;untreated group(15 cases with class 4,3 cases with class 3,2 cases with class 2)showed no obvious difference from model group, P >0.05;treated group(3 cases with normality,cases 10 with class 1,5 cases with class 2)was better than that of untreated group, P <0.05;treated group ALT(36.3U/L±11.5U/L),AST(46.7U/L±18.2U/L),γ GT(55.3U/L±12.3U/L),TNF α(1.6ng/ml±0.52ng/ml)were obviously lower than that of model and untreated groups(ALT1 98.1U/L±66.3U/L,189.0U/L±69.7U/L,AST 253.0U/L±62.1U/L,268.0U/L±60.5U/L,γ GT 156.6 U/L±38.7U/L,149.8U/L±38.1U/L,TNF α 2.8ng/ml±0.65ng/ml,2.7ng/ml±0.60ng/ml), P <0.05 or 0.001.Conclusion ALD can be cured by alprostadil by declining the level of TNF α and improving liver tissue.更多还原