【摘要】 目的:构建含人B7-2基因的绿色荧光蛋白表达载体pEGFP-C3-B7-2。方法:应用RT-PCR、巢式PCR方法从重症肌无力患者胸腺组织中扩增出人B7-2cDNA基因片段,经回收、纯化、酶切后,依次连接到质粒pGEM-T和pEGFP-C3上。结果与结论:酶切及测序证实成功构建了克隆载体pGEM-B7-2和真核绿色荧光表达载体pEGFP-C3-B7-2。更多还原

【Abstract】 Aim: To construct eukaryotic fluorescent expression vector pEGFP GAAB2 C3 GAAB2 B7 GAAB2 2. Methods: Human B7 GAAB2 2 cDNA in the thymus tissue of patients with myasthenia gravis was amplified using RT GAAB2 PCR and nested PCR. The amplified product was examined by electrophoresis and sequence determination. This fragment was inserted into pGEM GAAB2 T plasmid and pEGFP GAAB2 C3 fluorescent expression vector. Results and Conclusion: The restriction enzyme digestion and the sequence analysis showed that recombined cloning vector pGEM GAAB2 B7 GAAB2 2,and expression vector pEGFP GAAB2 C3 GAAB2 B7 GAAB2 2 had been successfully constructed.更多还原