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可溶性耐药相关钙结合蛋白的生物质谱分析与鉴定

Identification of Soluble Resistance-related Calcium Binding Protein by Bio-mass Spectrometric Analysis

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【作者】 刘宁齐静吕磊刘志强刘淑莹杨纯正

【Author】 LIU Ning~(1,2), QI Jing~3, L\(U¨\) Lei~1, LIU Zhi-Qiang~(1*), LIU Shu-Ying~1, YANG Chun-Zheng~2(1. Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;2. Institute of Life Science and Technology, Jilin University, Changchun 130021, China;3. State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300020, China)

【机构】 中国科学院长春应用化学研究所新药研究实验室中国医学科学院血液学研究所实验血液学国家重点实验室中国医学科学院血液学研究所实验血液学国家重点实验室 长春130022吉林大学生命科学技术研究所长春130021天津300020长春130022天津300020

【Abstract】 The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.

【基金】 国家自然科学基金(批准号:20273067)资助.
  • 【文献出处】 高等学校化学学报 ,Chemical Research In Chinese Universities , 编辑部邮箱 ,2005年06期
  • 【分类号】R733.7
  • 【被引频次】1
  • 【下载频次】215
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