节点文献
可溶性耐药相关钙结合蛋白的生物质谱分析与鉴定
Identification of Soluble Resistance-related Calcium Binding Protein by Bio-mass Spectrometric Analysis
【Abstract】 The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
【关键词】 可溶性耐药相关钙结合蛋白;
基质辅助激光解吸离子化飞行时间质谱;
二维凝胶电泳;
【Key words】 Soluble resistance-related calcium binding protein; Matrix-assisted laser desorption ionization time of flight mass spectrometry; Two-dimensional gel electrophoresis;
【Key words】 Soluble resistance-related calcium binding protein; Matrix-assisted laser desorption ionization time of flight mass spectrometry; Two-dimensional gel electrophoresis;
【基金】 国家自然科学基金(批准号:20273067)资助.
- 【文献出处】 高等学校化学学报 ,Chemical Research In Chinese Universities , 编辑部邮箱 ,2005年06期
- 【分类号】R733.7
- 【被引频次】1
- 【下载频次】215