【摘要】 氯酸钾抗性是区分籼粳分化的指标之一。本研究以珍汕97(ZS97)和日本晴(Nipponbare)所构建的回交群体BC4F2为材料,用2‰浓度的氯酸钾溶液对芽长约5mm的幼芽持续处理24h后,根据其发芽数和具次生根苗数筛选获得6份对氯酸钾抗性的导入系,用BC5F1进一步验证发现其中3份株系存在抗性分离。这3份株系平均含有两个外源导入片段,背景回复率平均为95.4%。结果表明,在水稻第1、6、11、12染色体上存在4个氯酸钾抗性基因。更多还原

【Abstract】 Potassium chlorate tolerance is one of the criteria differentiated japonica from indica. In this study, we analyzed the genetic bases of potassium chlorate tolerance in rice using 88 backcross inbred lines that derived from an indica Zhenshan97B (ZS97) and a japonica Nipponbare. Initial screening of the backcross inbred lines for potassium chlorate tolerance was carried out after the shoots around 5mm subjected to 2‰potassium chlorate solution for 24 hours. Based on seed germination rate and shoots with seminal roots, 6 lines with potassi-um chlorate tolerance were obtained. Three of the 6 lines were found with the tolerance segregation by the de-scendant test. Graphical genotyping further showed the three lines had two introgression segments and the genome recovery ratio of 95.4% on average, and four QTLs detected respectively on chromosomes 1, 6, 11 and 12 for the potassium chlorate tolerance.更多还原