【摘要】 猪繁殖与呼吸综合征是目前养猪业中一种严重的病毒性传染病,引起猪严重繁殖障碍和呼吸道疾病。该病的病原体属于动脉炎病毒属,是一种不分节段的单股正链RNA病毒,含有8 个开放阅读框(ORFs),ORF1 编码非结构蛋白,ORF2~ORF7 编码结构蛋白。其中ORF7 编码的核衣壳(N)蛋白和ORF6编码的非糖基化基质(M)蛋白为优势结构蛋白。猪繁殖与呼吸综合征病毒基因组存在广泛的遗传变异性,M蛋白和N蛋白在所有的毒株之间相对比较保守,可作为血清学诊断的靶抗原。文章就猪繁殖与呼吸综合征病毒在分子生物学方面的研究情况做作一综述。更多还原

【Abstract】 Porcine reproductive and respiratory sydrome (PRRS)is presently one of the most important viral infectious disease of swine, which is characterized by severe reproductive failure and respiratory distress. The viral etiology of PRRSV is PRRSV, which belongs to the Arteriviridae family and has a nonsegmented, single-stranded and positive-sense RNA genome. PRRSV consists of eight overlapping opening reading frames(ORFs)and ORF1 encodes the non-structual protein and the ORF2-ORF7 codes the structural proteins. Especially the nucleocapsid(N)protein encoded by ORF7 and the nonglycosylated membrane(M) protein encoded by ORF6 are the major structural proteins. The viral genetic variation exists widely and the M protein and the N protein are comparatively steady in all strains,which can be used as antigens for serological diagnosis.The article discussed some aspects about PRRSV researches on molecular biology.更多还原