【摘要】 为了探索利用植物分泌特性来表达重组蛋白的可行性,先构建了含钙网蛋白信号肽的植物双元载体pBI-cal,再向该载体中插入霍乱毒素B亚单位编码基因,最后得到表达载体pBIcal-ctb。通过根癌农杆菌介导,该表达载体转化烟草,在卡那霉素抗性培养基上筛选,得到30棵抗性植株。经PCR鉴定,霍乱毒素B亚单位基因已经整合到烟草基因组中。初步表达分析表明,转基因烟草中含有具生物活性的霍乱毒素B亚单位蛋白。更多还原

【Abstract】 Binary phyto-vector pBIcal of calreticulin was first constructed to probe the feasibility to use phyto-secretion to express recombinant proteins,the cholera toxin B subunit gene was then transferred into the vector,and expression vector pBIcal-ctb was finally obtained.By means of Agrobacterium tumefacions ,the expression vector was transferred into tobacco that was cultured on the kanamycin-resistant medium to get 20 resistant plants.PCR identification proved that cholera toxin B subunit gene had been integrated into tobacco genome.The primary analysis indicated the transgenic tobacco contained bioactive cholera toxin B subunit protein.更多还原