【摘要】 目的　探讨颗粒细胞上促卵泡素 (FSH -R)的含量与体外受精胚胎移植 (IVF -ET)结果的关系。方法　在 38个IVF周期中 ,将受精后获得的颗粒细胞 ,采用流式细胞测定仪测定其表面FSH -R的含量 ,并与IVF结果进行比较。结果　颗粒细胞上FSH -R的含量与获卵个数无显著的相关关系 (P >0 .0 5 )。受精率、卵裂率随颗粒细胞上FSH -R的含量增加而增加 (r =0 .5 7P <0 .0 1,r =0 .6 1P <0 .0 1)。妊娠组与非妊娠组的颗粒细胞上FSH -R含量有显著性差异 (P <0 .0 0 0 1)。结论　卵泡液中FSH与颗粒细胞膜上FSH -R相互作用共同促进卵母细胞的成熟和发育。更多还原

【Abstract】 Objective: Comparing the expression of follicle stimulating hormone receptor (FSH-R) on cultured granrlosa cells and the correlation between the expression and IVF outcome to give a rich theoretic base on controlled ovarian hyperstimulation. Methods: We studied 38 cycles of controlled ovarian hyperstimulation of patients with IVF-ET. After fertilization granulosa cells were isolated from oocytes and immediately sent to assess the expression of FSH-R on the granulose cells by FMC,and compare the correlation between the expression and IVF outcome. Results: The expression of FSH-R had no correlation with the numbers of oocyte retrival (P>0.05). The ratio of fertilization and cleavage had positively correlation with the expression of FSH-R(r=0.57 P<0.01, r=0.61,P<0.01). The expression of FSH-R on granulosa cells had significant difference between the pregnant cycles and nonpregnant cycles. The mean expression in the pregnant cycles was statistically significantly higher than that in the nonpregnant cycles (P<0.0001). Conclusions: The FSH in FF and FSH-R on granulosa cells react each other and increase the development and maturation of oocytes.更多还原