【摘要】 目的 :应用RP HPLC(DAD)建立了水蔓菁的指纹图谱。方法 :用BeckmanC18柱 (4 6mm× 2 5 0mm ,5μm ) ,甲醇 0 5 %冰醋酸溶液恒流洗脱 ,流速 1ml/min ,检测波长 2 5 4nm。结果 :获得了该药材HPLC指纹图谱共有模式 ,并对不同批药材进行了相似度比较。结论 :指纹图谱可用于控制水蔓菁的内在质量。更多还原

【Abstract】 Objective:To establish HPLC fingerprint of Herba Veronicae Dilatatae.Methods:Beckman C 18 column was used,with mixtures of 0.5% ice acetic acid-methanol as mobile phase.The flow rate was 1 ml/min.The wavelength of detection was 254 nm.Results:After the mutual mode of HPLC fingerprint was set up,comparsion of similar degree with the medicinal materials of different batch were carried out. Conclusion:According to the selected chromatographic conditions,a good fingerprint has been described.The method is simple,accurate and reproducible.It may have practical value for the quality control of samples of Herba Veronicae Dilatatae.更多还原