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腺嘌呤对菠萝快速繁殖的影响

The Effects of Adenine on Pineapple Macropropagation

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【作者】 詹儒林昝丽梅

【Author】 ZHAN Ru-Lin 2ZAN Li-Mei1(Southern Subtropical Crops Research Institute,Chinese Academy of Tropical AgriculturalSciences, Zhanjiang 524091)2(Hainan Tianxiang Biotechnologies,Limited Liability Company, Haikou 571101)

【机构】 中国热带农业科学院南亚热带作物研究所海南天香生物工程有限公司 湛江524091海口571101

【摘要】 以腺嘌呤(adenine,Ad)作为基本培养基(MS)的添加成分,进行菠萝的快速繁殖。以不加Ad作对照,结果显示不同浓度的Ad分别为1.0、 2.0、 3.0、 4.0、 5.0、 6.0 mg.L-1对芽的分化及增殖均无显著促进作用。而在壮苗培养中,除Ad为1.0 mg.L-1外,其他各浓度均能极显著地提高幼苗的生长速率,而且在不同浓度间也出现了极显著差异。Ad在3.0~5.0 mg.L-1时,幼苗生长速率最快,可使出苗时间提前30 d以上。

【Abstract】 Stem-tips of pineapple (Ananas comosus (L.) Merr.) were cultured on a modified MSmedium supplemented with various concentrations (1.0, 2.0, 3.0, 4.0, 5.0, 6.0 mg.L-1) of adenine (Ad).There were no significant effects on shoot culture and bud proliferation culture as compared with thecontrast (Ad-free MS medium). However, bud growth rate on the stage of elongation culture wassignificantly higher than the control, under the Ad concentrations more than 1.0 mg.L-1 mg in themedium.The results also showed that the concentrations (3.0~5.0 mg.L-1) of Ad are the best for budelongation. In these conditions, the period of elongation culture can be reduced 30 days at least.

【关键词】 腺嘌呤菠萝快速繁殖
【Key words】 AdenineAnanas comosus (L.) Merr.Macropropagation
  • 【文献出处】 植物学通报 ,Chinese Bulletin of Botany , 编辑部邮箱 ,2004年03期
  • 【分类号】S668.3
  • 【被引频次】5
  • 【下载频次】90
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