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腺嘌呤对菠萝快速繁殖的影响
The Effects of Adenine on Pineapple Macropropagation
【摘要】 以腺嘌呤(adenine,Ad)作为基本培养基(MS)的添加成分,进行菠萝的快速繁殖。以不加Ad作对照,结果显示不同浓度的Ad分别为1.0、 2.0、 3.0、 4.0、 5.0、 6.0 mg.L-1对芽的分化及增殖均无显著促进作用。而在壮苗培养中,除Ad为1.0 mg.L-1外,其他各浓度均能极显著地提高幼苗的生长速率,而且在不同浓度间也出现了极显著差异。Ad在3.0~5.0 mg.L-1时,幼苗生长速率最快,可使出苗时间提前30 d以上。
【Abstract】 Stem-tips of pineapple (Ananas comosus (L.) Merr.) were cultured on a modified MSmedium supplemented with various concentrations (1.0, 2.0, 3.0, 4.0, 5.0, 6.0 mg.L-1) of adenine (Ad).There were no significant effects on shoot culture and bud proliferation culture as compared with thecontrast (Ad-free MS medium). However, bud growth rate on the stage of elongation culture wassignificantly higher than the control, under the Ad concentrations more than 1.0 mg.L-1 mg in themedium.The results also showed that the concentrations (3.0~5.0 mg.L-1) of Ad are the best for budelongation. In these conditions, the period of elongation culture can be reduced 30 days at least.
- 【文献出处】 植物学通报 ,Chinese Bulletin of Botany , 编辑部邮箱 ,2004年03期
- 【分类号】S668.3
- 【被引频次】5
- 【下载频次】90