【摘要】 以麻疯树上胚轴为实验材料在 MS 添加 IBA和 BA 的培养基上进行离体培养实验。结果表明,在 IBA 0.1 mg/L 与 BA 0.2~0.7 mg/L 组合的条件下,不定芽从上胚轴外植体的表面直接被诱导分化,其中以在 MS+IBA 0.1 mg/L+BA 0.5 mg/L 上的诱导率最高。从愈伤组织来源的植株再生需要IBA 0.5 mg/L 与 BA 0.1 mg/L、IBA 0.5 mg/L 与BA 0.2 mg/L以及 IBA 1.0 mg/L与 BA 0.5 mg/L的激素组合,其分化的最佳培养基是MS+IBA1.0mg/L+BA0.5 mg/L。生长健壮的不定芽和再生植株能在无激素的 MS 基本培养基上生根。发育良好的再生苗可成功地转移到温室栽培而没有可见的变异。更多还原

【Abstract】 In vitro epicotyl explants from Jatro- pha curcas were cultured on MS medium with in- dole-3-butyric acid (IBA) and 6-benzyladenine (BA). Adventitious buds were directly induced from the surface of epicotyl explants under the condition with the combinations of IBA 0.1 mg/L and BA 0.2-0.7 mg/L. Of which IBA 0.1 mg/L and BA 0.5 mg/L induced the highest regenera- tion frequency. Shoot regeneration from callus re- quired the combinations of IBA 0.5 mg/L with BA 0.1 mg/L, IBA 0.5 mg/L with BA 0.2 mg/L and IBA 1.0 mg/L with BA 0.5 mg/L, and the most suitable combination was IBA 1.0 mg/L and BA 0.5 mg/L. The health adventitious buds and regen- erated shoots could be rooted on growth regula- tor-free MS medium. Regenerated plants with well developed shoots and roots were successfully transferred to greenhouse, without visible detect- able variation.更多还原