【摘要】 目的 :研究电磁脉冲 (EMP)辐照对白血病肿瘤细胞株Jurkat细胞分泌细胞因子IFN -γ和IL - 4的影响 ,探讨EMP对Jurkat细胞损伤机制。方法 :Jurkat细胞经EMP照射后即刻、1h、 6h、 12h、 2 4h、 4 8h、 72h共 7个时相点 ,细胞悬液甩片 ,4 %多聚甲醛 4℃固定 ,自然晾干 ,进行SP法的IFN -γ和IL - 4蛋白免疫组化染色。在光镜 10× 4 0倍视野下 ,应用CMIAS-H图像分析仪对阳性细胞的图像分析参数积分光密度值 (IOD)进行统计分析。结果 :免疫组化结果表明 ,场强 6× 10 4 V/m的电磁脉冲能使Jurkat细胞IFN -r表达持续性减少 ,差异非常显著 (P <0 0 1) ,而IL - 4变化不明显 (P >0 0 5 )。结论 :EMP辐照后Th1型的细胞因子IFN-γ明显减少 ,而Th2型的细胞因子IL - 4未见显著变化 ,提示 :EMP辐照后机体中受到影响的主要是细胞免疫 ,而体液免疫功能未受到明显影响更多还原

【Abstract】 Objective:With observing the changes of expression of IFN-γ and IL-4 in Jurkat cell after being exposure to electromagnetic to explore the possible injury mechanism Methods: The Jurkat cell were collected at instantly,1h, 6h,12h,24h,48h,72h respectively after exposure to EMP The expression of IFN-γ and IL-4 in Jurkat cells were determined by immunohistochemical SP method..stereological analysis for over 100.cells on the glass slide were performed by CMTAS-Ⅱ image analysis system at a magnification 400. ALL data was analyzed by SPSS8.0 software. The significance of differences among groups was analyzed with mono-factor analysis of variance. Results: IL-4 has not statistically change but IFN-r both has decreased. statistically .Conclusion cell mediated immunity maybe plays an important role in the injury of Jurkat cell by EMP.更多还原