【摘要】 【目的】 探讨胚胎干细胞源性的表皮干细胞在腹腔微环境中的分化情况,为研究其在不同微环境中的分化稳定性和全能性及寻找新的皮肤工程种子细胞奠定基础?【方法】 小鼠ES E14细胞与人羊膜共培养4～5 d,定向诱导其分化为表皮样干细胞克隆,它们呈β1整合素?CK15和CK19阳性,移植入裸鼠腹腔?对移植后细胞的分化情况进行形态学和CEA?CK10?CK18?CK19免疫组织化学观察?【结果】 小鼠胚胎干细胞源性表皮样干细胞在裸鼠腹腔内1～4周,细胞分化为由单层或复层上皮样细胞构成的管状和泡状结构?种植5周后,除上述结构外,可见角化复层扁平上皮?毛囊样?汗腺样及皮脂腺样等结构?免疫组化表明汗腺样结构分别呈CEA和CK18阳性,而角化复层扁平上皮的基底层细胞分别呈CK19和CK10阳性?【结论】 初步结果表明小鼠胚胎干细胞源性表皮样干细胞在腹腔微环境下同样具有分化为角化复层上皮?毛囊样?汗腺样和皮脂腺样结构的潜能?更多还原

【Abstract】 Objective To investigate the differentiation of the ES-derived epidermal stem cells in abdominal cavity microenvironment, to lay a basis for the study of stability and totipotence of epider-mal stem cells in different microenvironment, and to seek new source of seed cells for skin tissue engi-neering. Methods Mouse embryonic stem cells of E14 were cocultured with human amnion for 4-5 days. The clone of epidermal-like stem cells were formed with positive expression of β1 integrin, CK15 and CK19. The differentiation patterns of dornor cells were observed and estimated with morphologi-cal and CEA,CK10,CK18,CK19 immunohistochemical method. Results Within 1-4 weeks after trans-plantion into nude mice abdominal cavity, the ES-derived epidermal-like stem cells were differentiated into the tubular or follicular like structure lined with simple or stratified epithelium-like cells. After 5 weeks, keratinized stratified squamous epithelium, hair follicles-like, sweat glands-like and seba-ceous glands-like structure were observed. The sweat glands-like structure expressed CEA and CK18 respectively, the stratum basale of keratinized stratified squamous epithelium were CK19 and CK10 posi-tive staining. Conclusion The preliminary study suggests that the ES-derived epidermal-like stem cells have the differentiation potential to form the structure including keratinized stratified squamous epithelium, hair follicles-like, sweat glands-like and sebaceous glands-like structure in abdominal cavi-ty microenviorment.更多还原