【摘要】 目的 :不同时限腹主动脉缩窄造成后负荷增高型大鼠心室肥厚和心衰模型。方法 :Wistar大鼠随机分为A组 :腹主动脉缩窄 4周 ;B组 :腹主动脉缩窄 8周及假手术组。比较心室肥大指数 ,心肌组织镜下改变 ,血液动力学变化及模型达心衰时间。结果 :A组、B组心衰率分别为 16 .6 7%、73.6 8% ,大鼠死亡均发生在术后 1周内 ;A组MBP、LVSP升高 ,B组HR、MBP、LVSP及±dp/dt下降、LVEDP升高 (P <0 .0 1～ 0 .0 5 ) ;A、B组LVMI及RVMI分别升高 16 .9%、2 7.8% ,和 19.8%、4 0 .7% (P <0 .0 1～ 0 .0 5 ) ,两组间无差异 (P >0 .0 5 ) ;光镜下见心肌纤维排列紊乱 ,小血管壁增厚 ,炎症细胞浸润 ,心肌细胞肥大 ,间质增生。结论 :Wistar大鼠腹主动脉缩窄术后 4周可达高血压心室肥厚模型 ,8周可达心衰模型 ,手术应激可能是大鼠死亡的主要原因 ;腹主动脉缩窄大鼠发生心室重构 ,心室重构早于心衰的发生 ,建议尽早开始逆转心室肥厚治疗。更多还原

【Abstract】 Objective:To establish rat model of ventricular hypertrophy and heart failure by partially banding abdominal aorta for different time limits.Methods:60 Wistar rats were randomly divided into three groups:A group which were ligated for 4 weeks;B group being ligated for 8 weeks;and sham-operated group (each containing 20 Wistar rats).Left and right ventricular mass index,hemodynamic parameters,cardiac morphological changes and the time of achieving heart failure model were investigated in different groups.Results:There were 16.67% heart failure rats in A group,while 73.68% in B group.The death of models occurred in the first week after experimental procedure.MBP and LVSP were increased greatly in A group,and HR,MBP,LVSP and ±dp/dt were decreased while LVEDP increased in B group compared with A and sham-operated groups( P <0.01～0.05).LVMI and RVMI were risen up by 16.9% and27.8%,19.8% and 40.7% respectively in A and B groups( P <0.01～0.05).No significant difference about ventricular mass index existed in A and B rats. Under optical microscope,turbulent cardiomyocytes,hypertrophic myocytes and thickoned small vessel wall were seen in experimental rats compared with sham-operated group.Conclusion:Hypertensive hypertrophy and heart failure model will be achieved by partially banding abdominal aorta for 4weeks,8 weeks,respectively.And the main reason of death of experimental rats may be operation stress.Ventricular remodelling,which exists in aortic banding rats,is prior to occurrence of heart failure.It is suggested that the therapy of reversing ventricular hypertrophy should be given as early as possible.更多还原