【摘要】 目的 :观察核因子NF -κB在大鼠急性梗阻性胆管炎 (acuteobstructivecholangitis ,AOC)所致多器官损害中的作用。方法 :4 2只Wistar大鼠随机分为AOC组、单纯胆总管结扎组 (BDL组 )和假手术组 (SO组 ) ,于术后 6h和 4 8h活杀 ,取肝、心、肺和肾脏组织观察形态学改变 ,检测上述组织中NF -κB活性 ,作血气分析 ,测定血清乳酸脱氢酶 (LDH)、谷丙转胺酶 (ALT)、尿素氮 (BUN)和肌酐 (Cr)水平 ,以及血浆中肿瘤坏死因子 (TNF) -α和白介素 (IL) - 6含量。结果 :AOC组动物的肝、心、肺和肾脏组织出现显著病理改变 ,各组织中NF -kB活性较其它组显著增强。AOC组血气分析结果明显异常 ,与其它组有显著差异 (P <0 .0 5 )。AOC组的血清LDH、ALT、BUN及Cr水平以及血浆中TNF -α、IL - 6含量明显高于其它组 (P <0 .0 5 )。结论 :在AOC期间 ,肝、心、肺和肾组织中NF -κB活性明显增强 ,同时伴有组织病理改变和器官功能损害 ,提示NF -κB激活在AOC所致多器官损害中起重要作用更多还原

【Abstract】 Objective:To investigate the role of NF-kB activation in the development of multiple organ dysfunction (MOD) during acute obstructive cholangitis (AOC) in rats.Methods:42 Wistar rats were divided into three groups:the AOC group,the group of bile duct ligation (BDL group),and the sham operation group (SO group).All the animals were killed at 6th h or 48th h after operation.Morphological changes in vital organs were observed under light and electron microscope.NF-κB activation was determined with Electrophoretic Mobility Shift Assay (EMSA).Arterial blood gas analysis was performed.Other parameters included the serum levels of lactate dehydrogenase (LDH),alanine aminotransferase (ALT),blood urea nitrogen (BUN),creatinine (Cr) and the plasma levels of TNF-α and IL-6.Results: The significant changesinhistology and ultrastructure of vitalorgans were observedin AOC group .By contrast ,inBDLgroup ,allthefeaturesoforgandamageweregreatlyreduced .NF -κBactivationinvarioustissues markedlyin creased in AOC group when comparedwith other two groups.Inaddition ,there sults of bloodgasanalysis were obviously abnormalin AOC group ,and there was asignificant difference between AOC an do the rgroups (P <0 .0 5 ) .Moreover,in AOC group ,these rumlevels of LDH ,ALT ,BUN and Cr and the plasmalevels of TNF -αandIL - 6 were signifi cantly higher than those of other groups (P <0 . 0 5 ) .Conclusion :TheNF -κBactivation occursin manyvitalorgans during AOC ,which is as so ciated with histopat hological changes an dorgan dy sfunction ,so NF -κBact ivation may play an import antrole in the development of MOD duringAOC .更多还原