【摘要】 目的 :观察血竭总黄酮对小鼠三叉神经节 (TG)细胞河豚毒素敏感型 (TTX- S)钠通道电流的影响 ,确定血竭对 TG细胞电压门控性钠通道电流产生抑制效应的有效部位 .方法 :酶解法急性分离昆明种小鼠 (1月龄 )三叉神经节细胞 ,应用全细胞膜片钳技术记录 TG细胞 TTX- S钠通道电流 ,观察血竭总黄酮对其影响 .结果 :在钳制电位为- 90 m V时 ,3种浓度 (0 .0 0 1% ,0 .0 1% ,0 .1% )的血竭总黄酮对锋钠电流的抑制率分别为 17.0 2± 5 .4 2 % ,33.2 3± 5 .12 % ,4 9.30± 5 .14 % (P值均小于 0 .0 5 ) ,抑制效应呈典型的浓度依赖性 ,血竭总黄酮对 TG细胞 TTX- S锋钠电流的半数抑制浓度 (IC50 )为 0 .10 13% .结论 :血竭总黄酮是血竭抑制 TG细胞 TTX- S钠通道电流的有效部位 ,该研究为血竭有效成分的提取缩小了研究范围更多还原

【Abstract】 The purpose of our present work is to search out the effective fraction of Resina Draconis on TTX-sensitive voltage-gated sodium currents（VGSC） in TG neurons. The effect of Flavone in Resina Draconis（FRD） on TTX-sensitive VGSC in TG neurons were observed. The TG neurons were enzymatically isolated from mice. The effect of FRD on the TTX-sensitive VGSC was assessed by using whole-cell patch-clamp technique.When the holding potential was -90 mV,the three concentrations of FRD（0.001 %,0.01 %,0.1 %） reduced peak sodium currents by 17.02±5.43 %,33.23±5.12 % ,49.30±5.14 %（P<0.05）,with a IC₅₀ （50 % inhibiting concentration） of 0.101 3 %. FRD significantly inhibit the TTX-sensitive peak sodium currents in a dose-dependent manner. It is concluded that FRD is the effective fraction of Resina Draconis in inhibiting TTX-sensitive VGSC in TG neurons. Our present work reduces the range to extract the effective ingredients of Resina Draconis.更多还原