【摘要】 用AFLP、REP-PCR、23SrDNAPCR-RFLP等方法对分离自四川的23株葛藤属的根瘤菌的遗传特性进行了研究。结果表明,供试的葛藤根瘤菌遗传特性存在明显差异。AFLP分析结果显示,23个菌株分为7个AFLP遗传群;在BOXAIR-PCR分析中,23株根瘤菌则被分为5个遗传群;对23SrDNAPCR-RFLP分析表明,葛藤根瘤菌分属于中慢生根瘤菌属(Mesorhizobium)、慢生根瘤菌属(Bradyrhizobium)2个属,聚类分析结果将其划分为5个遗传群。分离自四川省的葛藤根瘤菌存在较大的遗传多样性。更多还原

【Abstract】 Twenty-three rhizobial strains isolated from Pueraria spp. in different sites of Sichuan province were studied by using AFLP, BOXAIR-PCR and 23S rDNA PCR-RFLP techniques. The results showed that genetic diversity among these rhizobia was existed. The analysis of AFLP revealed that, there were seven AFLP genotypes among these rhizobial strains; and BOXAIR-PCR suggested that, there were five genotypes existed. The results from 23S rDNA PCR-RFLP analysis revealed that, from the phylogeny point of view, these rhizobia belong to Mesorhizobium sp. and Bradyrhizobium sp., and however, most of them were different from the type strains of the related genera, which indicated that maybe there are putative new species existed.更多还原