【摘要】 以自交不亲和(self-incompatibility,　SI)甘蓝为材料,研究了自花授粉和异花授粉过程中甘蓝花柱内蛋白激酶的动态变化和该激酶与多种离子的关系。结果表明,甘蓝自花授粉和异花授粉后3　min时,蛋白质磷酸化活性有显著差别。Ca2+、Mn2+、Mg2+都是磷酸化活性充分发挥所必需的,添加 Ca2+浓度以 2　mmol·L-1为最佳浓度,Mg2+和Mn2+在此磷酸化活性发挥中可能起协同作用,它们同时存在能极大地提高磷酸化活性,乙二醇双(2-氨基乙醚)四乙酸[ethyleneglycol　bis(2-aminoethylether)tetraacetic　acid,EGTA]使蛋白磷酸化活性大大降低,该激酶对钙调素(calmodulin,CaM)没有依赖性。更多还原

【Abstract】 Dynamic changes of the activity of protein kinase in style of Brassica oleracea L. in self-pollination and cross-pollination process and the relationship between the activity of protein kinase and various ions were studied. The results showed that the activity of protein kinase was significantly different after 3 min between self-pollination and cross- pollination. The activity of protein kinase increased by addition of Ca2+, Mn2+, Mg2+. The optimum concentration of added calcium ion was 2 mmol·L-1. It is speculated that due to their synergetic effects, the coexistence of Mg2+ and Mn2+ increased the activity of protein kinase significantly. However, addition of EGTA decreased the activity of protein kinase. This kind of protein kinase has no dependence on CaM.更多还原