【摘要】 目的探讨eNOS基因转移防治移植静脉血管桥再狭窄的可行性。方法构建带有eNOS基因的复制缺陷型重组腺病毒载体(AdCMVeNOS)和腺病毒空载体(AdCMV)。取山羊颈静脉作血管桥,在体外转染AdCMVeNOS(实验组)和AdCMV(对照组),然后旁路移植到自体颈动脉上。术后30d免疫组化染色,测定静脉桥3H-TDR的掺入量和静脉桥内膜厚度及管腔狭窄面积百分比,观察eNOS基因在静脉桥中的功能表达和静脉桥新生内膜的增生情况。结果转染后30d,实验组静脉桥中外源性eNOS仍有功能表达;静脉桥3H-TDR掺入量与对照组相比明显减少(P<0.01);静脉桥平均内膜增生厚度和管腔狭窄面积百分比明显低于对照组(P<0.01)。结论eNOS基因转移可以有效抑制移植血管新生内膜的增生,对预防移植血管的狭窄有一定作用。更多还原

【Abstract】 Objective To determine the possibility that eNOS gene transfer prevents restenosis of vein grafts.Methods Constructed the recombinant adenovirus vector AdCMVeNOS and AdCMV.Jugulars of goat were taken as grafts,infected AdCMVeNOS and AdCMV in vitro,then anastomosed the vein grafts between carotids of goats.Immunohistochemical staining was used to asses the functional expression of eNOS in vein grafts.The inhibition of intimal hyperplasia in vein grafts transducted AdCMVeNOS was assesed using assay of 3H-TDR incorporation,histologic analysis,measursment of intimal thickness and percent area stenosis of vein grafts.Results There has been expression of eNOS gene in vein grafts30days after transduced with Ad-CMVeNOS.Levels of 3H-TDR Incorporation:Incorporation in AdCMV veins was487.7±51.1cmp /mg vessel,and140.8±32.5cmp /mg vessel in AdCMVeNOS veins(P<0.01,n=3).Mean thickness of intimal was125.7±30.60(m in AdCMV veins)and28.8±5.24(m in AdCMVeNOS veins);Percent area stenosis was(52.18±8.46)%in AdCMV veins,and(23.6±4.71)%in AdCMVeNOS veins.Conclusion Adenovirus-mediated eNOS gene transfer could inhibit intimal hyperlasia effectively.This work lay a foundation in gene therapy of grafts resteno-sis after coronary artery bypass grafting(CABG).[更多还原