【摘要】 目的　研究表达轮状病毒主要抗原VP7、VP6的非复制型重组腺病毒在恒河猴模型上的免疫反应性和安全性 ,为轮状病毒基因工程疫苗的进一步研究奠定基础。方法　将重组腺病毒通过滴鼻或灌胃途径免疫恒河猴 ,用酶联免疫吸附实验 (ELISA)检测恒河猴血清中轮状病毒特异性抗体IgG ,并监测免疫前后各组恒河猴血、尿常规及一系列反映肝功能和肾功能的生化指标。在整个实验过程中 ,常规监测恒河猴的体温、体重及临床症状。结果　重组腺病毒通过滴鼻或灌胃途径免疫均能诱导恒河猴产生针对轮状病毒抗原的特异性血清IgG ;在重组腺病毒免疫整个过程中 ,各免疫组恒河猴无不良反应 ,体温基本正常 ,体重增长正常 ;和正常对照组相比 ,免疫后各免疫组恒河猴血、尿常规及肝功能、肾功能无显著性变化。结论　表达轮状病毒抗原的重组腺病毒免疫恒河猴能产生针对轮状病毒的特异性免疫反应 ,并且不影响其正常的生长发育及健康状况 ,初步证明我们制备的重组腺病毒用作疫苗的有效性和安全性更多还原

【Abstract】 Objective To preliminarily evaluate the immunity and safety of the recombinant adenoviruses expressing rotavirus structural proteins VP7 and VP6 in rhesus monkeys to lay a foundation for the development of novel genetic engineering vaccine against rotavirus. Methods Baby monkeys were immunized with the recombinant adenoviruses intranasally or orally. Serum IgG against rotavirus was measured with ELISA. During the course of the immunization, besides the daily monitoring of body temperature, weight and clinical symptoms, the routine blood and urine tests and liver and kidney function tests were also conducted. Results Monkeys immunized via intranasal or oral routes could both generate serum IgG against rotavirus. During the immunization, the temperature of monkeys was normal and body weight raise stably. Both routine blood and urine tests and liver and kidney function tests showed no significant alteration compared with the control group. Conclusion The immunization with the recombinant adenoviruses expressing rotavirus antigens is able to induce rotavirus specific efficient immune responses and is safe to baby rhesus monkeys. The preliminary results implied that the recombinant adenoviruses could be an ideal vaccine for rotavirus and lay a foundation for further studies.更多还原