【摘要】 目的　了解佛山市外环境水体中非O1群霍乱弧菌的菌型分布及毒素携带情况。方法收集外环境水样 ,用常规的细菌培养方法分离非O1群霍乱弧菌。采用分子生物学PCR技术检测霍乱肠毒素 (CT)基因 ,用ELISA检测耐热肠毒素 (ST)和不耐热肠毒素 (LT)。结果　共检测水样 16 4 4份 ,分离到非O1群霍乱弧菌 4 78株 ,分离率为 2 9 0 7% ;血清学分型显示近年佛山市外环境水体中非O1群霍乱弧菌的主要菌型为VBO7。霍乱肠毒素基因检测阳性率为 1 91% ;耐热肠毒素、不耐热肠毒素的检出率分别为 13 14 %和 12 17%。结论　个别非O1群霍乱弧菌可同时带有多种毒力因子 ,提示佛山市外环境水体中存在的非O1群霍乱弧菌具有潜在的致病性 ,加强监测对做好感染性腹泻的防治工作实属必要。更多还原

【Abstract】 Objective To study the distribution of serotype and the positive rate of toxins among vibrio cholerae non-O₁ isolated from environmental waters in Foshan city. Methods Water specimens were collected from river and cultured for vibrio cholerae non-O₁. The PCR method was used to detect cholerae enterotoxin （CT） gene; the ELISA method was used to detect heat-stable toxin （ST） and heat-labile toxin （LT）. Results 478 vibrio cholerae non-O₁ strains were isolated from 1 644 water specimens, with a positive rate of 29.07%. Serological assay showed that the main serotype of vibrio cholerae non-O₁ in Foshan city is VBO₇. Positive rate of CT, ST and LT were 1.91%, 13.14% and 12.17%, respectively. Conclusions A few non-O₁ strains were found to have several virulent factors simultaneously, and the results suggest that vibrio cholerae non-O₁ in environmental waters is potentially pathogenic and may affect people’s health.It is necessary to pay attention to the prevention of diarrhoea caused by vibrio cholerae non-O₁.更多还原