【摘要】 目的　研究结核分枝杆菌异烟肼耐药菌株与敏感菌株的蛋白质表达差异 ,鉴定结核分枝杆菌中与异烟肼耐药相关的菌体蛋白。方法　应用双向电泳技术比较 2株异烟肼耐药菌株与 2株敏感菌株的全菌蛋白表达图谱差异 ,发现异烟肼耐药结核分枝杆菌表达量显著增加 2 6个蛋白质斑点 ,通过基质辅助激光解吸 /电离飞行时间质谱仪进行质谱分析 ,获得 6个明确的肽质量指纹谱 ,通过蛋白质数据库进行检索分析。结果　 6个蛋白分别为海藻糖磷酸磷酸酶、铁钼蛋白A、莽草酸 5脱氢酶、葡萄糖胺果糖 6磷酸氨基转移酶、吲哚 3甘油磷酸合成酶、TetR家族转录调控因子。结论　上述蛋白的鉴定将对发现新型抗结核药物靶位和异烟肼耐药结核病诊断的分子标志物可能有所裨益更多还原

【Abstract】 Objective To compare the proteome of isoniazid(INH)-resistant M. tuberculosis with that of sensitive strains for identifing unique proteins of these strains. Methods The two-dimensional electrophoresis(2-DE) were used to compare the global protein patterns between INH-resistant and INH-sensitive strains. Protein spots remarkly increased in INH-resistant strains, which were digested in gel by enzyme and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry( MALDI-TOF-MS). The data obtained from peptide mass fingerprinting were used in protein database search. Results Six spots of proteins in gel were identified as trehalose-6-phosphate phosphatase, moaA, shikimate 5-dehydrogenase, glucosamine-fructose-6-phosphate aminotransferase, indole-3-glycerol phosphate synthase and TetR-family transcriptional regulator. Conclusion These proteins are potentially target sites for chemotherapy against M. tuberculosis and new marker in the detection of resistance of M. tuberculosis .更多还原