【摘要】 目的　探讨 β 链接素 (β cat)和基质金属蛋白酶 7(MMP 7)表达与结直肠癌发生发展和浸润转移的关系。方法　制作结直肠腺瘤 腺癌组织芯片 ,免疫组织化学检测 β cat和MMP 7在结直肠腺瘤 腺癌组织的表达。结果　 (1) β cat胞核异常表达率在腺瘤癌变组织 (35 9% )显著高于腺瘤(16 7% )及腺癌组织 (19 7% ,P <0 0 5 )。 (2 )腺瘤伴重度异型增生者 β cat胞质、胞核异常表达率显著高于轻度者 (P <0 0 5 )。 (3)溃疡型、淋巴结转移阳性及C D期腺癌组 β cat胞核异常表达率均显著高于隆起型、淋巴结阴性及A B期组 (P <0 0 5或P <0 0 1)。 (4)MMP 7阳性表达率在腺癌组织(6 9 2 % )显著高于正常黏膜 (15 0 % )、腺瘤 (35 0 % )及癌变组织 (46 2 % ,P <0 0 5或P <0 0 1)。(5 )溃疡型、淋巴结转移阳性及C D期腺癌组MMP 7阳性表达率均显著高于隆起型、淋巴结阴性及A B期腺癌组 (P <0 0 5 )。 (6 ) β cat胞质、胞核异常表达与MMP 7阳性表达显著正相关 (P <0 0 1)。结论　β cat胞质及胞核异常表达与结直肠癌的发生密切相关 ,可能是结直肠癌发生的早期事件 ;同时 β cat还可能通过胞核易位增强其下游靶基因MMP 7的降解作用促进结直肠癌浸润转移。更多还原

【Abstract】 Objective To investigate the relationship between β catenin and matrix metalloproteinase 7 (MMP 7) expression and development / biologic behavior of human colorectal cancer.Methods Immunohistochemical study for β catenin and MMP 7 was carried out on colorectal adenoma carcinoma tissue microarrays and results analyzed.Results The nuclear β catenin expression rate was 35 9% in adenoma with malignant transformation, significantly higher than that in adenoma (16 7%) and carcinoma (19 7%) (both P <0 05). The cytoplasmic and nuclear β catenin expression rate in adenoma with severe dysplasia was significantly higher than that in adenoma with mild dysplasia (both P <0 05). The nuclear β catenin expression rate in adenocarcinomas of the ulcerative type, with lymph node metastasis and in the late tumor stages were all significantly higher than that in adenocarcinomas of the polypoid type, with negative lymph node and in the early tumor stages ( P <0 05 or P <0 01). The MMP 7 expression rate in adenocarcinoma (69 2%) was significantly higher than that in normal colorectal mucosa (15 0%), adenoma (35 0%) and adenoma with malignant transformation (46 2%, P <0 05 or P <0 01). The MMP 7 expression rate in ulcerative type adenocarcinoma with lymph node metastasis and in late tumor stages was significantly higher than that in polypoid type adenocarcinoma with negative lymph node and in early tumor stages (all P <0 05). The cytoplasmic and nuclear β catenin expression was thus in positive correlation with the expression of MMP 7 (both P <0 01).Conclusions The cytoplasmic and nuclear β catenin expression, probably an early event, was related to the development of colorectal cancer. β catenin may enhance the degradative function of the target gene MMP 7 through nuclear translocationand may further facilitate local invasion and metastasis by the colorectal cancer cells.更多还原