【摘要】 目的 :从丹参中筛选血管紧张素转换酶抑制剂 (ACEI)。方法 :分别提取丹参水溶性和脂溶性部分 ,用荧光检测法筛选抑制血管紧张素转换酶 (ACE)活性的有效部位或成分。结果 :丹参水溶性部分、丹参总酚酸及丹酚酸B显著降低了大鼠肺组织的ACE活性 ,其IC50 分别为 ( 2 .4 5± 0 .0 7) ,( 0 .2 4± 0 .0 2 ) ,( 0 .0 2± 0 .0 1)g·L-1。含丹参酮Ⅰ ,Ⅱ的菲醌类成分或脂溶性部分没有作用。 结论 :丹参具有ACEI作用 ,其活性成分存在水溶性部分中 ,主要为含丹酚酸B等酚酸类物质更多还原

【Abstract】 Objective:To screen angiotensin converting enzyme inhibitor (ACEI) from traditional Chinese medicine, Salvia miltiorrhiza . Method: Hydrophilic and lipophilic fractions of S. miltiorrhiza were isolated, and their effective components were screened by a fluorimetric assay for inhibition of angiotensin converting enzyme (ACE). Result: Water soluble fractions, total salvianolic acids and salvianolic acid B markedly decreased ACE activity of rabbit lung tissue. Their IC 50 value were( 2.45±0.07),(0.24±0.02 ),(0.02±0.01) g·L -1 respectively. Lipophilic components or phenanthraquinones including tanshinone Ⅰand Ⅱ showed no changes on the activity of ACE. Conclusion: S. miltiorrhiza inhibits angiotensin converting enzyme and its active components are in aqueous extract, in which the main were salvianolic acids including salvianolic acids B.更多还原