【摘要】 目的 :建立了一种简单的环孢素A(CsA)的萃取方法和反相高液相色谱测定法。方法 :全血乙醚 石油醚 (7∶1 )单步提取后 ,经正己烷脱脂后进样 ;以C1 8为固定相 ,凹形梯度洗脱 ,(A液 :乙腈 水 (44∶1 1 ,内含 0 .0 2 %TFA) ,B液 :乙腈 ,1 2min内从A变化到B ,凹形梯度 ) ,检测波长 2 1 4nm ,柱温 5 5～ 70℃。结果 :CsA在 0 .0 5～ 2 0mg·L-1 范围内呈良好的线性关系。结论 :此方法提取步骤少 ,方法简单快速、专一性强、费用低 ,适合临床CsA的血药浓度监测 ,并测定了 1例NHLⅢB期患者非清髓性异基因干细胞移植后早期全血C2 浓度。更多还原

【Abstract】 OBJECTIVE A new simple extraction of cyclosporin A in whole blood and an improved method of HPLC were developed. METHODS CsA was extracted from the whole blood by ethyl ether: petroleum ether(4∶1),degreased by hexane, then analyzed in C 18 column by concave gradient (solution A: acetonitrile-water(44∶11) with 0.02% TFA; solution B: aetonitrile, A was transformed to B within 12 min by concave gradient).CsA was detected in 214 nm with CsD as internal standard. The column temperature was 55～70℃. RESULTS The method is linear within the range of 0.05～20 mg·L -1.CONCLUSION The method is simple, rapid, sensitive and applied to determine the therapeutic drug monitoring. The C 2 in a NHL ⅢB patient after nonmyeloablative alleogentic peripheral blood stem cell transplantation was determined.更多还原