【摘要】 目的 :探讨CGRP受体拮抗剂CGRP8 37对甲醛炎性痛大鼠自发痛反应及脊髓后角NOS表达和NO含量的影响。方法 :大鼠足底注射甲醛制造炎性痛模型 ;计数缩足反射次数反映自发痛程度 ;NADPH d组织化学法观察脊髓后角NOS表达 ;硝酸还原酶法测定NO-3 /NO-2 含量以反映NO含量。结果 :足底注射甲醛后 ,动物出现自发痛反应行为。足底注射甲醛后 2 4h ,双侧脊髓后角NOS表达及NO含量明显增加。预先鞘内注射CGRP8 37可使甲醛诱导的自发性缩足反射次数明显减少 ,并可明显抑制甲醛炎性痛诱导的脊髓后角NOS表达及NO含量的增加。结论 :甲醛炎性痛时 ,脊髓后角CGRP受体激活可促进NOS活性表达及NO的产生更多还原

【Abstract】 Aim: To study the effect of CGRP receptor antagonist CGRP8-37 on nociceptive response and expression of nitric oxide synthase （NOS） and content of nitric oxide（NO） in the dorsal horn of the spinal cord of rats during formalin-induced inflammatory pain. Methods: Using formalin injection into right hind paw induced inflammatory pain. Counting the times of flinching reflex was used to observe the degree of spontaneous pain. NADPH-d histochemistry was used to observe the changes of NOS expression. The content of NO was observed by measuring the contents of nitrate/nitrite（NO^-₃/ NO^-₂）. Results: spontaneous pain behavioral was elicited by formalin injection.The NOS expression and NO content significantly increased in the spinal cord at 24h after formalin injection. Intrathecal injection of CGRP8-37 could significantly inhibit the response of spontaneous pain and the increases of NOS expression and NO content induced by formalin injection. Conclusion: The activation of CGRP receptors enhances NOS expression and NO production in the dorsal horn of the spinal cord during formalin-induced inflammatory pain.更多还原