【摘要】 目的 观察不同人脑胶质瘤细胞的放射敏感性,测定种植系数（PE）和存活分数（SF）等参数并且根据L-Q模型绘制剂量存活曲线,为脑胶质瘤的综合治疗提供重要的参考资料。方法实验采用体外传代培养的11种人脑胶质瘤细胞株,即T98G、SF295、Skmg-1、Skmg-4、MGR-1、MGR-2、MGR-3、UWR-7、SF767、SHG-44和uw28。应用集落形成技术和L-Q模型确定放射敏感性参数及绘制剂量存活曲线。结果11种人脑胶质瘤细胞株的放射敏感性存在较大差异,PE从1.61％到40.89％;SF₂的范围从0.32到0.59,平均为0.44±0.10;SF₈从0.00到3.71×10^-2,平均为0.42×10^-2±1.1×10^-2。结论 人脑胶质瘤细胞具有不同的内在放射敏感性。更多还原

【Abstract】 Objective To investigate the intrinsic radiosensitivity of 11 glioma cell lines in vitro. Methods Eleven glioma cell lines, i.e. T98G, SF295, Skmg-1, Skmg-4, MGR-1, MGR-2, MGR-3, UWR-7, SF767, SHG-44 and uw28 were used in this study. The intrinsic radiosensitivity parameters of 11 human glioma cell lines were determined in vitro by clonogenic assay after different doses of X-irradiation （0～8Gy）. The survival curves were generated and mathematically analysed by using the L-Q model to obtain the radiosensitivity parameters, alpha, beta, SF₂ （the surviving fraction at 2 Gy） and SF-8 （the surviving fraction at 8 Gy）. Results There were great differences in the radiosensitivity among 11 glioma cell lines. For 11 glioma cell lines, the planting efficiency varied from 1.61% to 40.89%. The mean SF₂ was 0.44±0.10 with range of 0.32 to 0.59 and the mean SF₈ was 0.42×10^-2±1.1×10^-2 with range of 0.00 to 3.71×10^-2. Conclusion The intrinsic radiosensitivity varies in different human glioma cells lines.更多还原