【摘要】 目的 :探讨全反式维甲酸对细胞周期素依赖激酶抑制蛋白P2 7蛋白表达 ,血管平滑肌细胞DNA合成和增生的影响。方法 :取Wistar大鼠胸主动脉血管平滑肌细胞培养 ,用3H TdR掺入率检测血管平滑肌细胞DNA合成和增生 ,用western blotting印迹和图像分析方法检测细胞周期素依赖激酶抑制蛋白P2 7蛋白表达。结果 :全反式维甲酸 (2 5 μmol/L)明显抑制胎牛血清 (2 0 %FBS)诱导的血管平滑肌细胞P2 7蛋白表达 ;全反式维甲酸明显抑制胎牛血清诱导的血管平滑肌细胞增生和DNA合成。结论 :全反式维甲酸具有抗血管平滑肌细胞增生作用 ,其机制与促进P2 7蛋白表达有关更多还原

【Abstract】 Objective To investigate the effects of all-trans retinoic acid(ATRA)on proliferation of rat vascular smooth muscle cells and the expression of Cyclin-Dependent Kinase Inhibitor(CKI)P27 protein.Methods Cell culture of vascular smooth muscle cell of Wistar rat thoracic aorta, 3H-TdR incorporation,western blotting and picture analysation have been used.Results ATRA has attenuated the downregulation of CKI P27 protein induced by 20% fetal bovine serum.ATRA has also inhibited significantly DNA synthesis and proliferation of VSMCs stimulated by 20% fetal bovine serum.Conclusions ATRA has inhibited proliferation of VSMCs with the mechanism related to promoting the expression of P27 protein.更多还原