【摘要】 目的探讨一氧化氮(NO)、一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)、可溶性Fas(sFas)诱导大骨节病发生发展的作用与特点,为大骨节病防治研究提供新的思路。方法选择陕西省永寿县、榆林市榆阳区大骨节病区与咸阳市渭城区非大骨节病区120例调查对象,分为成人、儿童大骨节病组;成人、儿童病区对照组;成人、儿童非病区对照组,每组20例,平均年龄、性别无差别。用酶还原法检测了血清NO,化学比色法检测了血清NOS、iNOS,ELISA法检测了血清sFas水平。结果①大骨节病成人组血清NO、iNOS显著高于病区和非病区成人对照组(P< 0.05);血清NOS、sFas/APO鄄1水平高于非病区成人对照组(P< 0.005)。②大骨节病儿童组血清NO高于非病区儿童对照组(P< 0.05);血清NOS、iNOS均高于病区和非病区儿童对照组(P< 0.005)。结论儿童大骨节病发生发展中NO诱导途径起一定的作用,而Fas途径作用不明显。当疾病发展到成人大骨节病时,二者均起一定作用。更多还原

【Abstract】 Objective To investigate the induced effect of nitric oxide, NO synthase, and sFas/APO-1 in serum in the pathogenesis of Kashin-Beck disease(KBD) in order to probe a new strategy to control the disease. Methods 120 subjects, from KBD areas of YongShou and YuYang counties, and non-KBD areas of XianYang city, ShaanXi province, were divided into 6 groups:(1) the adult group with KBD, (2) the child group with KBD, (3) the adult control in KBD areas, (4) the child control in KBD areas, (5) the adult control in no-KBD areas, (6) the child control in no-KBD areas. The sex and the average age in the different groups were not different. The contents of nitric oxide, NO synthase, and sFas/APO-1 in serum of 6 groups were detected by method of nitrifying ferment, chemic colorimetry and ELISA, respectively. Results ① The contents of NO and iNOS in serum of the adult group with KBD were significantly higher than those of the adult control in KBD areas and no-KBD areas(P < 0.05). The contents of NOS and sFas/APO-1 in serum of the adult group with KBD were significantly higher than those of the adult control group in no-KBD areas (P < 0.005). ② The contents of NO in serum of the child KBD group were significantly higher than those of the child control in no-KBD areas (P < 0.05). The contents of NO and iNOS in serum of the child group with KBD were significantly higher than those of the child control group in KBD and no-KBD areas (P < 0.005). Conclusion NO induced way may plays a role in the pathogenesis in KBD children, but not sFas, and both NO and Fas induced ways have some effects in the KBD development with age.更多还原