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FL-1多功能吸附树脂对沙棘叶总黄酮的分离纯化
Separation and purification of total flavonoids from Hippophae rhamnoides leaves with FL-1 multifunctional resin
【摘要】 目的 考察 FL- 1多功能吸附树脂对沙棘叶中总黄酮的分离纯化方法。方法 根据沙棘叶黄酮的结构特征 ,考察了 FL - 1树脂的吸附性能 ,并采用高效液相色谱法对沙棘叶总黄酮进行了定量分析。结果 FL - 1树脂对沙棘叶总黄酮具有较高的吸附选择性 ,70 %乙醇作为脱附剂 ,产品中总黄酮含量为 4 0 .2 %。结论 FL- 1树脂用于沙棘叶总黄酮的分离纯化简便有效。
【Abstract】 Object To study the method for separating and purifying the total flavonoids in Hippophae rhamnoides leaves (TFH) with multifunctional resin FL-1. Methods According to structural characteristic of the flavone, FL-1 resin was systematically studied for its adsorption capability. HPLC was used to measure the content of TFH. Results FL-1 resin can be used to produce the TFH with high quality. The purity of the TFH is 40.2% in the dried part of 70% ethanolic elution. Conclusion It is a simple and efficient method to separate the TFH from H. rhamnoides leaves with FL-1 resin.
【Key words】 Hippophae rhamnoides L. leaves; total flavonoides; multifunctional resin FL-1; HPLC;
- 【文献出处】 中草药 ,Chinese Traditional and Herbal Drugs , 编辑部邮箱 ,2004年12期
- 【分类号】R284
- 【被引频次】17
- 【下载频次】372