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HPLC测定不同产地丹参药材中脂溶性成分

Determination of fat-soluble component in Radix Salviae miltiorrhizae from different sources

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【作者】 安睿王新宏周思丽尤丽莎王智华洪筱坤王依群

【Author】 AN Rui 1, WANG Xin hong 1, ZHOU Si li 1, YOU Li sha, WANG Zhi hua 1, HONG Xiao kun 1, WANG Yi qun 2 (1. Department of Chemistry, Shanghai University of TCM, Shanghai 201203, China; 2. Shanghai Huayu Pharmaceuntical Co., Ltd. Shanghai 200002, China)

【机构】 上海中医药大学化学教研室上海华宇药业有限公司 上海201203上海201203上海200002

【摘要】 目的 :研究不同产地丹参中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA 含量和测定方法。方法 :用HPLC法测定 ,以 1%醋酸甲醇溶液和 1%醋酸水溶液为流动相作梯度洗脱 ,检测波长为 2 80nm。结果 :所测 4种成分的平均回收率均大于 97% ,RSD小于 1.2 % ,线性良好。结论 :本分析方法稳定可靠 ,重现性好。

【Abstract】 AIM: To study the contents and determination method of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in Danshen from different sources. METHODS: Determination by HPLC with the gradient eluent consisted of methanol (1% acetic acid) water (1% acetic acid). The detection wavelength was at 280 nm. RESULTS: The average recovery of four components were all above 97%, RSD was no more than 1.2%. The calibration curve was linear. CONCLUSION: The method is accurate and reliable with good reproducibility.

  • 【文献出处】 中成药 ,Chinese Traditional Patent Medicine , 编辑部邮箱 ,2004年04期
  • 【分类号】R284
  • 【被引频次】30
  • 【下载频次】379
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