【摘要】 目的 :研究局灶性脑缺血大鼠在不同缺血时间和不同再灌注时间的脑梗塞体积比、皮质半影区葡萄糖转运体 1(GLUT1)转录水平和蛋白水平的表达。方法 :用线栓法复制大鼠局灶性脑缺血模型 ,用KontronIBAS2 5全自动图像分析系统进行图像分析脑梗塞体积比 ;剥取缺血半影区皮质组织 ,采用反转录 -聚合酶链反应 (RT -PCR) ,测定GLUT1mRNA水平的变化 ;用免疫组织化学半定量测定GLUT1蛋白水平的变化。结果 :脑缺血 1h后再灌注脑梗塞体积明显小于缺血 3h再灌注。GLUT1mRNA自 1h即开始升高 ,2 4h到达高峰 ,1周时仍高于假手术对照组 ;缺血 3h再灌注组在 3h开始升高 ,2 4h到高峰 ,1周后仍高于正常 ,但升高幅度不及MCAO 1h/R组。MCAO 1h/R组GLUT1蛋白水平自 3h开始升高 ,2 4h达到高峰 ,1周时接近正常 ;MCAO 3h/R组GLUT1在 3h下降 ,继之升高 ,2 4h到高峰 ,1周时接近正常。结论 :GLUT1在缺血半影区的表达上调 ,可能是机体对缺血损伤的保护性反应更多还原

【Abstract】 AIM: To investigate the volume percentage of infarct and expression of glucose transporter-1 (GLUT1) transcription and protein levels at different ischemic time point and different reperfusion time point in rat focal cerebral ischemic penumbra. METHODS: Focal ischemic models of middle cerebral artery occlusion (MCAO) in rats were made by inserting nylon thread. Brain samples were harvested from ischemic penumbra. Infarct volume were analyzed quantitively by Kontron IBAS 2.5 image auto-analyses system. The expressien of GLUT1 mRNA was assessed by RT-PCR, and the expression of GLUT1 protein was assessed by immunohistochemistry. RESULTS: The infarction volume in MCAO 1 h/reperfusion (R) group was obviously smaller than that in MCAO 3 h/R group. GLUT1 increased at (1 h) MCAO 1 h/R group, climbed to climax at 24 h and remained higher than normal at 1 week. In contrast, in the MCAO 3 h/R group, the corresponding index was at 3 h, 24 h and 1 week, but the increasing degree of GLUT1 was slighter than MCAO 1 h/R. GLUT1 protein began to ascend at 1 h, reached climax at 24 h and was higher than normal at 1 week in MCAO 1 h/R group, while in MCAO 3 h/R group, the corresponding index was at 3 h, 24 h and 1 week. CONCLUSION: GLUT1 expression is notably up-regulated in the penumbra region after focal cerebral ischemia, it may be a protective reaction against ischemic injury. [更多还原