【摘要】 目的 :探讨睡眠剥夺大鼠丝裂素活化蛋白激酶 (MAPKs)表达的变化及可能的意义。方法 :采用TUNEL和HE法观察了睡眠剥夺大鼠海马神经元的形态学变化 ,采用Westernblot法、β -液闪计数法观察海马神经元ERK和JNK表达的变化。结果 :快眼动睡眠剥夺组海马神经元阳性凋亡细胞数增多 ,ERK活性 176 4 0 0± 94 1 5 6 ,显著低于对照组 (P <0 .0 5 ) ,快眼动睡眠剥夺组JNK蛋白表达量为 87 5 % ,显著高于对照组 (P <0 0 5 )。结论 :睡眠剥夺可引起大鼠海马神经元MAPKs活性的变化 ,而这些变化可能涉及神经元的凋亡机制更多还原

【Abstract】 AIM: To explore the change and the possible role of MAPKs in rat hippocampus neuron after sleep deprivation. METHODS: The morphology of hippocampus neuron after sleep derivation was observed by TUNEL and HE staining, the activity of ERK was assayed by β-liquid scintillation counting and the expression of JNK was detected by Western blot. RESULTS: In paradoxical sleep deprivation (PSD) group, the number of apoptotic cells in hippocampus was increased. The scores of ERK activity were (1 764.00)±941.56. Compared with control groups, the ERK activity was obviously decreased (P<0.05). The JNK expression was 87.5%, which was higher than that in control group. CONCLUSION: These results provide some important evidences that the sleep deprivation could cause changes in MAPKs activity, which may be related to the mechanism of hippocampus neuron apoptosis. [更多还原