【摘要】 目的 :探讨caveolin - 1蛋白在VSMC的分布、在ET - 1诱导的VSMC增殖中表达的变化情况。方法 :在培养的VSMC上 ,使用 [3 H]TdR掺入法观察ET - 1和BQ12 3对其DNA合成的影响 ;用荧光免疫组化方法观察cave olin - 1蛋白在VSMC的分布 ,ET - 1对caveolin - 1蛋白表达的影响 ;用Westernblot检测ET - 1和BQ12 3对caveolin - 1蛋白表达的影响。结果 :ET - 1可以呈浓度梯度地刺激VSMC的增殖 ,ETAR特异性阻断剂BQ12 3可以抑制ET - 1的作用 ;免疫荧光证实了在VSMC上 ,caveolin - 1分布于细胞表面 ,在ET - 1刺激增殖过程中 ,VSMC上caveolin - 1蛋白荧光强度下降 ;Westernblot证实ET - 1可以抑制caveolin - 1蛋白的表达。结论 :在VSMC上 ,caveolin - 1主要分布于细胞表面 ,在ET - 1刺激增殖过程中 ,caveolin - 1蛋白表达发生了变化 ,ET - 1可通过ETAR抑制caveolin - 1蛋白的表达。更多还原

【Abstract】 AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The -thymidine (TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS: The ETA receptor specific antagonist BQ123 inhibited the increase in TdR incorporation in response to ET-1 on VSMC. Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC. After 24 h treatment of VSMC with ET-1, the expression of caveolin-1 in VSMC was significantly decreased. Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC, BQ123 reversed the effect of ET-1. CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor.更多还原