【摘要】 概述了研究土壤微生物多样性的主要方法 .传统上 ,土壤微生物群落的分析依赖于培养技术 ,使用各种培养基最大限度地培养各种微生物群体 ,但仍只能培养和分离出一小部分土壤微生物群落 .使用Biolog分析、磷脂脂肪酸分析和核酸分析等方法 ,可研究和表征那些现在还不能够被培养的土壤微生物 ,从而获取关于土壤微生物群落多样性的更多和更完整的信息 .更多还原

【Abstract】 This paper gave a review on the main methods for studying soil microbial diversity.Traditionally,the analysis of soil microbial communities relied on culturing techniques,using a variety of culture media.However,only a small fraction of the soil microbial community has been cultured and isolated with this approach.Other methods such as Biolog GN analysis,phospholipids fatty acids analysis and nucleic acid-based analysis can be used to study and characterize soil microbes which currently cannot be cultured,and to get more and complete information about soil microbial community.更多还原