【摘要】 <正> 作为核酸基本结构单位的核苷酸,代谢活跃,具有重要的生物功能,是核酸研究中受重视的领域之一。胞苷-5′-磷酸(5′-CMP)具有明显的药理作用,茜素红S(ARS)作为探针对蛋白质的研究已有报道,本研究发现在pH=4.56的条件下,5′-CMP可使ARS的UV-Vis光谱最大吸收峰红移90 nm。据此,ARS作为5′-CMP的光谱探针,建立新的研究体系,获得5′-CMP与小分子探针反应的结合数、浓度线性范围等的有关数据。该方法灵敏度高,选择性好,在一定条件下可作为5′-CMP的定量方法。更多还原

【Abstract】 The binding reaction of cytidine 5′-phosphate(5′-CMP) with Alizarin Red S(ARS) was studied in the buffer solution(pH=4.56) as functions of temperature, time, ionic strength and interference of inorganic substance, biological substance and surfactant by UV-Vis spectrophotometry. It was found that the bathochromic shift of the maximum absorption peak of the complex was 90nm. The apparemt molar absorptivity was ε=3.80x10~3 L/(mol·cm), the binding number was n=13, and the linear range of reaction was 0～14 mg/L.更多还原