【摘要】 目的　研究阿米洛利 (amiloride)对豚鼠心肌细胞钾电流及钙电流的作用。方法　采用全细胞膜片钳技术记录豚鼠心室肌细胞钾通道及钙通道电流。结果　阿米洛利在 10 10 0 μmol·L- 1 抑制L型及T型钙电流 ,不改变钙电流I-V曲线的形状 ,仅抑制这两型电流的幅度。当累积浓度达 10 0 μmol·L- 1 时 ,阿米洛利轻微抑制快激活延迟整流钾电流 (IKr) ,对慢激活延迟整流钾电流 (IKs)无影响。阿米洛利在 110 0 μmol·L- 1 浓度依赖性地抑制内向整流钾电流(IK1 )。结论　阿米洛利抑制电压依赖性的钾、钙电流 ,为其抗心律失常作用提供了离子基础更多还原

【Abstract】 Aim To elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na +/H + exchanger inhibitoramiloride. Methods Single ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques. Results Exposure to amiloride (10-100 μmol·L -1), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak I Ca,L, But amiloride did not change the shape of their I-V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 μmol·L -1 amiloride,I Kr was slightly depressed and I Ksdid not change. Amiloride (1-100 μmol·L -1) depressed I K1 in a concentration-dependent manner. Conclusion Amiloride depressed potassium and calcium currents,which may give support to its uses in some diseases of the cardiovascular system.更多还原