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水稻芽鞘紫线抑制与反抑制现象的遗传及反抑制基因的SSR分析

Analysis on the Inheritance of Inhibition and Anti-inhibition of Coleoptile Purple Line in Rice and the SSR Location of A (i(t)) Gene

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【作者】 张毅沈福成杨光伟何光华张正圣杨正林

【Author】 ZHANG Yi,SHEN Fu-Cheng,YANG Guang-Wei~①, HE Guang-Hua~①,ZHANG Zheng-Sheng, YANG Zheng-Lin (Key Open Laboratory of Biotechnology and Crop Quality Improvement of Agricultural Ministry, Southwest Agricultural Vniversity,Chongqing 400716,China)

【机构】 西南农业大学农业部生物技术与作物品质改良重点开放实验室西南农业大学农业部生物技术与作物品质改良重点开放实验室 重庆400716重庆400716重庆400716

【摘要】 从芽鞘有紫线和无紫线的杂交组合中发现 6个组合的F2 代紫线有无比例为 13∶3,以无紫线且为不育系的亲本XNA为母本对组合XNA/ 2 1A15 0进行回交 ,BF1代的分离比例符合 1∶1。遗传分析表明 :水稻芽鞘紫线表达受抑制基因I和反抑制基因Ai(t) 的影响 ,I抑制芽鞘紫线C_A_P_系统中的P基因 ,Ai(t) 抑制I基因。以BF1构建了Ai(t) 基因的定位群体 ,SSR分析表明 :Ai(t) 基因与RM 335、RM 2 95、RM2 87、RM 2 1连锁 ,其遗传距离分别为2 8cM、10 2cM、13 9cM、2 6 1cM。

【Abstract】 The ratio of purple line :no-purple line(13∶3) was observed in six different F 2 populations produced by crossing between parents with purple line and no-purple line in coleoptile. The backcross of XNA//XNA/21A150 (XNA,no-purple line and CMS,as the recurrent parent) resulted in a ratio of 1∶1 (purple line∶no-purple line). Genetic analysis showed that the expression of rice coleoptile purple line was influenced by two genes,inhibiting gene I and anti-inhibiting gene A (i(t)). I gene inhibits P gene of C_A_P_ system and A (i(t)) inhibits I gene,respectively. The gene pools of A (i(t)) a (i(t)) and a (i(t)) a (i(t)) were constructed with BF 1 of XNA//XNA/21A150. SSR analysis indicated that A (i(t)) gene was linked with the markers of RM335,RM295,RM287 and RM21 and the genetic distance from A (i(t)) to these four markers were 2.8 cM,10.2 cM,13.9 cM,26.1 cM,respectively.

【基金】 国家自然科学基金 (编号 :3 0 2 0 0 173 )资助~~
  • 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,2004年08期
  • 【分类号】S511
  • 【被引频次】6
  • 【下载频次】105
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