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Ce4+对东北红豆杉细胞有丝分裂的影响
Effect of Ce4+ on the Mitosis in Taxus Cuspidata Cell Culture
【摘要】 以悬浮培养的东北红豆杉细胞为材料,研究了不同浓度的Ce4+在不同处理时间对细胞有丝分裂的影响。结果显示,Ce4+能诱发东北红豆杉细胞多种异常有丝分裂,在浓度为0.5mmol/L、处理时间为5h时,即开始出现异常有丝分裂现象,而且细胞有丝分裂指数(MI)比对照组明显降低(P<0.05),表明细胞有丝分裂受到抑制,Ce4+对细胞有丝分裂过程有显著影响。Ce4+具有一定遗传毒性。
【Abstract】 Effects of Ce4+ on the Taxus cuspidata cells in suspension culture were studied. The cells were treated with a series of concentration of Ce4+ for 5h,18h,42h,66h and 90h . The results showed that treated with 0.5mmol/L Ce4+ for 5h , the mitotic index (MI) could begin to be obviously decreased(P<0.05), and Ce4+ could cause abnormal cells mitosis. It suggested Ce4+ could inhibit the mitosis of the Taxus cuspidata cells. Ce4+ have weak genetic toxicity.
【关键词】 Ce4+;
东北红豆杉细胞;
有丝分裂指数;
异常有丝分裂;
【Key words】 Ce4+; Taxus cuspidata; mitotic index; abnormal mitosis;
【Key words】 Ce4+; Taxus cuspidata; mitotic index; abnormal mitosis;
【基金】 国家自然科学基金重点项目(20236040)
- 【文献出处】 稀土 ,Chinese Rare Earths , 编辑部邮箱 ,2004年04期
- 【分类号】Q942
- 【被引频次】5
- 【下载频次】111