【摘要】 [目的]探讨熊果酸对人乳腺癌细胞(MCF鄄7)增殖抑制和诱导凋亡的作用,以及凋亡发生与细胞内Ca2+浓度([Ca2+]i)变化的关系。[方法]5～40μmol/L浓度梯度的熊果酸处理MCF鄄7细胞24小时,用MTT比色法测定细胞增殖能力。10μmol/L,20μmol/L和30μmol/L熊果酸处理细胞24小时,用末端脱氧核苷转移酶介导dUTP末端标记法和AnnexinV流式细胞仪法检测凋亡细胞。20μmol/L熊果酸处理细胞24小时,用Fura鄄2荧光负载方法测定[Ca2+]i的变化。[结果]从20μmol/L熊果酸浓度起对MCF鄄7细胞的增殖抑制率显著升高(P<0.01),呈剂量依赖性,半增殖抑制浓度(IC50)为36.18μmol/L。20μmol/L和30μmol/L熊果酸使细胞凋亡率显著升高(P<0.01)。熊果酸处理组的[Ca2+]i明显高于对照组(P<0.05)。[结论]熊果酸具有抑制MCF鄄7细胞增殖和诱导凋亡的作用;其诱导凋亡可能依赖于细胞内Ca2+水平上调。更多还原

【Abstract】 To investigate the effects of ursolic acid(UA) on proliferative inhibition, apoptotic induction, and the relationship between UA-induced apoptosis and intracellular Ca2+ concentration in human breast carcinoma(MCF-7) cells. After MCF-7 cells treated with UA in the concentrations from 5μmol/L to 40μmol/L UA for 24h, cell viability of proliferation was assessed by MTT assay. Treated MCF-7 cells with 10μmol/L, 20μmol/L, and 30μmol/L UA for 24h, the rates of cell apoptosis were examined by terminal deoxynucleotide transferase mediated dUTP nick-end-labeling method and flow cytometry with Annexin V/ propidium iodide fluorescent stain. Treated with 20μmol/L UA for 24h, intracellular Ca2+ concentration was measured by Fure-2 fluorescein load method. Treated with the concentrations from 10μmol/L to 40μmol/L UA, the rates of proliferative inhibition were increased significantly(P<0.01). The degrees of the inhibition were in a dose-dependent fashion. IC50 of the proliferative inhibition was 36.18μmol/L. Treated with 10μmol/L, 20μmol/L and 30μmol/L UA, the rates of cell apoptosis were increased significantly(P<0.01). Intracellular Ca2+ concentration after treatment with UA was significantly higher than that of control(P<0.05). [Conclusion] Ursolic acid has the effects of inhibition to MCF-7 cell proliferation and induction of cell apoptosis. The apoptosis induced by UA may depend on rise of intracellular Ca2+ level in MCF-7 cells.更多还原