【摘要】 目的:探讨人胚脑源性神经干细胞特性及免疫原性,为神经干细胞(neuralstemcells,NSCs)移植治疗神经系统损伤奠定基础。方法:取孕14周流产胚胎,无菌分离制备大脑神经细胞悬液,用CD133标记的免疫磁珠分选NSC,提取NSCRNA,进行RT-PCR探测MHC-I,MHC-II类分子mRNA转录;用流式细胞术检测细胞表面MHC-I,MHC-II类分子的表达;用正常成人外周血单个核细胞与培养NSC共培养和MTT比色分析法观察淋巴细胞增殖反应;将NSC小鼠脑内接种,进行组织染色观察细胞移植部位的组织学变化。结果:新分离和传代的NSC均发生MHC-I,MHC-II类分子mRNA转录,MHC-I类分子在NSC表面的表达百分率为(5.66±0.37)%,NSC在体外能诱发淋巴细胞增殖反应,A值为1.09±0.48和1.15±0.29,与阴性对照比差异有显著性意义(P<0.05)。NSC小鼠脑内移植具有修复神经损伤作用,局部无淋巴细胞浸润和炎症。结论:人胚脑源性神经干细胞具有免疫原性,但直接脑内接种未发现引起明显的移植排斥反应。更多还原

【Abstract】 AIM: To explore the characteristics and immunogenicity of neural stem cells (NSCs) derived from human embryonic brain tissue, so as to provide evidence for the treatment of nervous system injury by transplantation of NSCs. METHODS: Abortion embryo of 14 weeks after pregnancy, were sterilely isolated to prepare cerebral neural cell suspension, and then the NSCs were isolated from human embryonic brain tissue, RNA of NSCs was extracted by using immunomagnetic beads labeled with CD133. The mRNA transcriptions of major histocompatibility complex I (MHC I) and MHC II molecule were detected by reverse transcription polymerase chain reaction(RT PCR), and the expressions of MHC I and MHC II molecule were measured by flow cytometry. The proliferation of lymphocytes stimulated by NSCs was evaluated by MTT colorimetry. Expanded NSCs were grafted into the brain of Balb/c mice and lymphocytes infiltrating were evaluated with histological examination. RESULTS: Both isolated and expanded NSCs expressed MHC I and MHC II molecules mRNA transcription, and the percentage of NSCs expression on the cell surface was (5.66±0.37)%. NSCs could induce proliferation reaction of lymphocytes in vitro, the A values were 1.09±0.48 and 1.15±0.29, significantly different from those of negative controls(P< 0.05). The NSCs transplanted into the brain of mice could repair the neural injury, and no lymphocytes and inflammation cells were observed in the grafted brain tissue of mice. CONCLUSION: The NSCs derived from human embryonic brain tissue possesses immunogenicity, but no obvious transplanted reject reaction is observed after direct inoculation into the brain.更多还原